Part:BBa_K243008:Design

Middle Linker-Fok_a

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 517
  

Design Notes

The middle length of the linker is useful to produce a stable connection to another part and to create a certain distance. The middle distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_a to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domains Fok_a and Fok_i.
Designed according to the RFC 25.

Commented GenBank file

Source

Combinated by cloning.